Use of media and serum in clinical culture of mesenchymal stromal cells

by Alexey Bersenev on March 4, 2015 · 1 comment

in cell culture, mesenchymal

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Last year I was trying to capture and analyze results of all published clinical studies in cell-based regenerative medicine. One dimension that I was able to capture is using of media and serum for mesenchymal stromal cell (MSC) clinical culture. Today, I’d like to share some data.

You can read about the methodology of this analysis here.
Out of total 116 regenerative medicine clinical studies, 45 used MSC culture. I was able to capture information about media/serum use from 36 studies out of 45. I did not have access to 4 studies and the other 5 studies did not describe MSC culture method.

Cell culture media
Some studies did not specify cell culture media. DMEM (including DMEM/F12) was the most popular:


25 studies of 36 used animal serum (FBS) and 11 studies used replacements of animal serum. 1 study did not specify any cell culture media supplements. 1 study was utilizing 2 animal serum replacements.


Serum was only FBS. The most popular FBS concentration was 10%


Finally, I looked at animal serum replacements. 1 study use animal replacement – bovine serum albumin (BSA), the other 10 used human products:


How to cite:
Bersenev Alexey. Use of media and serum in clinical culture of mesenchymal stromal cells. StemCellAssays blog. March 14, 2015. Available:

{ 1 comment… read it below or add one }

Daniel September 17, 2016 at 12:12 am

Your research is really interesting. Currently I´m working with stem cell and I have a concern about the use of FBS on stem cell cultures for clinical applications. Do you know if there is a special regulation about it?

Thank you


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