Methods and Protocols Digest – October 2014

by Alexey Bersenev on October 27, 2014 · 0 comments

in methods, protocols

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Methods and Protocols Digest is a collection of links to the methodological articles, freely available online. Here you can also share your lab protocol with community!


General lab
Commercial antibodies and their validation (F1000 Research)
Why do you have low cell number after FACS sorting? (Bitsize Bio)
Tips for a Happily Functioning Tissue Culture Room (Bitsize Bio)
10 Things Smart Scientists Do Before Sorting Cells (Excyte)

Pluripotent stem cells
Animal components-free human embryonic stem cell culture – review (Stem Cells TM)
Generation of lung epithelium from murine pluripotent stem cells in 3D culture (Stem Cells Dev)
Testing spinner flask for iPS cell culture (PLoS ONE)
Generation of human chondroprogenitors from embryonic stem cells for cartilage repair (Stem Cells TM)
Efficient generation of retinal pigment epithelium from human pluripotent stem cells (Stem Cells TM)

Neural cells
Generation of adult limbal neurosphere cells (PLoS ONE)
Derivation of mature neurons from human embryonic stem cells (F100 Research)
Neurotrophic requirements of human pluripotent stem cell-derived motor neurons (PLoS ONE)

Endothelial cells
High density dot culture for bone marrow-derived endothelial progenitors (PLoS ONE)

Mesenchymal stromal cells
Umbilical cord Wharton’s jelly MSC repeated culture system (PLoS ONE)

Tissue engineering
Generation of human iPS cell-engineered cardiac tissue sheets (Sci Reports)

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