Methods and Protocols Digest – September 2014

by Alexey Bersenev on September 22, 2014 · 0 comments

in cytometry, methods, protocols

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Methods and Protocols Digest is a collection of links to the methodological articles, freely available online. Please share your protocol with community!

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Organoids
Common organoid-initiating cells for mouse pancreas and liver (Stem Cell Res)
Pancreatic organogenesis – self-organization from stem cells in 3D (Stem Cells)

Direct reprogramming
Efficient generation of induced neurons from human fibroblasts (Sci Reports)

Pluripotent stem cells
Purification of iPS cell intermediates from a reprogrammable mouse model (JoVE)
Improved generation of mouse iPS cells in knockout serum replacement medium (PLoS ONE)
Isolation of mouse trophoblast stem cells in defined culture conditions (PLoS ONE)

Cardiac cells
Propagation of ES cell-derived cardiomyocytes in a defined microcarrier spinner culture (Stem Cell Res Ther)
Bioluminescent imaging of iPS cell-derived cardiomyocytes in infarcted heart (PLoS ONE)

Hematopoiesis
Angptl3 promotes expansion of expansion of murine hematopoietic stem cells ex vivo (PLoS ONE)
iPS-based model of Down syndrome-associated haematopoiesis abnormalities (Sci Reports)

Cytometry
Cell surface profiling using high-throughput flow cytometry (PLoS ONE)
Five controls for flow cytometry (BitsizeBio)

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