Analysis of MSC clinical preparation protocols

by Alexey Bersenev on February 19, 2014 · 0 comments

in mesenchymal, reviews

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The recent review analyzes published mesenchymal stromal cells (MSC) preparations for clinical trials. 47 clinical protocols were analyzed, published from 2007 to 2013. I’d encourage you to read full text! A few bullet points as a teaser:

  • Processing included MNC isolation from bone marrow by ficoll in 62%, by percoll in 9%, the whole unseparated marrow was used in 16%.
  • The most commonly used labware for primary cell seeding: “Corning (27%) and Falcon (27%) equally, followed by Nunc (23%), Greiner (18%)”.
  • “The most commonly used seeding density of BM MNC: 1.5-1.6×105 cells/cm2“; it varied extremely: from 1.1×103 to 1.0×106 cells/cm2.
  • The same labware brands were used for MSC expansion as for isolation: Corning (35%), Nunc (25%), Falcon (20%), Greiner (20%).
  • The most frequent basal media for MSC expansion: “DMEM-low glucose (53%), DMEM (15%), and αMEM (11%)”.
  • As for serum and analogs were used: 10% FBS – 73%, 15-20% FBS – 5%, platelet lysate – 11%, other – 11%.
  • The most frequently used plating density in passaging was 3-6×106 cells/cm2.

Good review!

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