Synthetic surfaces for pluripotent stem cell culture

by Alexey Bersenev on December 6, 2013 · 1 comment

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I’d like to share new and very interesting review, published in Cell Regeneration. The authors address the question of pluripotent stem cell interaction with surfaced used in cell culture via adhesion molecules. The field significantly progressed in the last decade by moving from feeder cells to extracellular matrices (ECM) and chemically defined (synthetic) surfaces.
Some surfaces, mentioned in review:

  • Integrins
  • Cadherins
  • Collagens
  • Laminins
  • Fibronectin
  • Vitronectin
  • Proteoglycans
  • Polymer
  • Hydrogels

The review includes such commercially available products as: Synthemax (Corning), StemAdhere (Primorigen), Celtrex (Invitrogen), Matrigel (BD) and other.
The authors also compiled the table for cost comparison of commercially available surfaces. I’d like to quote just a small piece about synthetic surfaces (emphasis mine):

Furthermore, synthetic surfaces are being developed based on the functional subunits of ECMPs and these surfaces have been reported to maintain pluripotency through more specific interactions (see Table 4 for examples). Defined surfaces thought to specifically interact with hPSCs through different CAMs appear to be equally supportive of culture, although direct and detailed comparisons between hPSCs maintained on surfaces that interact with different CAMs have not been performed.

This is the most comprehensive review on the subject. I’d highly recommend it to everyone, who culture ES- or iPS cells.

{ 1 comment… read it below or add one }

Andrew Laslett December 7, 2013 at 8:16 am

Thanks Alexey, we tried to write it so that it would be useful both for stem cell researchers and surface chemists.

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