Webinar: Standardization of CFU assay for hematopoietic progenitor cells

by Alexey Bersenev on April 2, 2013 · 1 comment

in cord blood, educational, hematopoietic, notes

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We’ve written a lot about significance of Colony Forming Unit (CFU) assay standardization. Today, I’d like to share a webinar on this topic. Stephen Szilvassy is Associate Director of Hematopoietic Product R&D at STEMCELL Technologies Inc. The webinar focuses on use of CFU in cord blood banking and transplantation.

The webinar will review several ways in which the CFU assay has been standardized, including the use of the STEMvision™ platform for automated scoring of all of the major CFU sub-types in different hematopoietic tissues.

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Jeanne Adiwinata Pawitan May 18, 2014 at 6:35 am

Dear Dr. Szilvassy,
I have seen the webinar, but I do not understand the slide titled: stemvission differentially scores colonies produced by erythroid….

There are orange, red, yellow and blue circles. How do you grouped the colonies into CFU/BFU-E, CFU-G/M/GM and CFU-GEMM? They all look the same for me. Moreover, in the right upper square, the colony in blue circle, it seems that there are one big, one middle size and two small colonies, it is right? or they are regarded as one colony?

Thanks a lot in advance

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