Synthetic feeder mimetics for pluripotent stem cell culture

by Alexey Bersenev on October 20, 2012 · 1 comment

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Development of synthetic substrates is very important for clinical-grade feeder-free and xeno-free culture of pluripotent stem cells (PSC). We touched these issues last year. Today I’d like to share an excellent review – The Evolution of Human Pluripotent Stem Cell Culture: from Feeder Cells to Synthetic Coatings.

In the first part of review, the authors describe the risks, associated with feeder cells and highlight the necessity for defined conditions and development of feeder-free culture. Interestingly, they highlight disadvantages of MatrigelTM (the most commonly used substrate in research) in human stem cell therapies:

However, MatrigelTM is derived from Engelbreth-Holm-Swarm mouse sarcomas [14], exhibits lot-to-lot variability and can introduce unwanted xenogeneic contaminants. Therefore, MatrigelTM is not an ideal substrate for feeder-free culture of hPSCs if the primary objective is to culture these cells for eventual human therapies.

Because biological substrates can not be clinically compliant, synthetic feeder mimetics were developed. The authors described desired properties of synthetic clinically-grade substrates, such as: ability to support self-renewal and proliferation of stem cells, scalability, low cost, re-usability:

At this time, only SynthemaxTM and PMEDSAH have shown compatibility with common sterilization methods [32, 33, 38]. Furthermore, the inclusion of peptides and proteins in stem cell culture substrates may not allow the surfaces to be reused because biomolecules are known to undergo degradation from metalloproteinases secreted by cultured cells [39]. This observation should also be considered as a factor for future clinical adoption, as the inclusion of biomolecules leads to increased costs.

Great review! Highly recommended!
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reporteras Milon August 19, 2013 at 1:41 am

not only good but also subjective article. thanks. feeder free stem cells
Alstem, based in Richmond, California specializes in iPSC generation services, viral packaging services and stem cell freezing medium.

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