Membrane filtration for rapid enrichment of adipose-derived stem cells

by Alexey Bersenev on September 21, 2012 · 1 comment

in adipose, methods

Post to Twitter Send Gmail Post to LinkedIn

Enrichment and isolation of stem cell populations from adipose tissue is a promising and rapidly developing area of cell therapy. The usual processing scheme includes adipose tissue dissociation (usually by enzymes) and derivation of so-called stromal vascular fraction (SVF). SVF is enriched for stem/ progenitor cell populations (including mesenchymal stromal cells – MSC) and could be used fresh at point-of-care or undergo subsequent cell culture for 1-3 weeks. The big questions is the difference in quality and therapeutic potency of fresh SVF versus cultured expanded MSC. If we can purify MSC from adipose tissue more efficiently, we may not need to culture SVF at all. In this case we can save on a labor, time and cost of therapy.

A new research paper, published in Biomaterials describes a protocol for purification of human adipose-derives stem cells (ADSCs) from SVF, which based on membrane filtration:

… we purified hADSCs from a digested solution of human adipose tissue by the conventional cell culture method and by the membrane filtration method using unmodified polyurethane (PU) membranes and surface-modified PU membranes, and we compared the purities and the osteoblast differentiation abilities of the hADSCs after each purification.

The authors isolated ADSCs from SVF by further filtration or by conventional 1-2-week cell culture and compared their identity and osteogenic potency:

The cells in the recovery solution had a 3- to 4.5-fold higher level of expression of the mesenchymal surface markers (8% vs. 24–38%) than the primary adipose tissue cells did (Fig. 6). However, the cells in the recovery solution did not express the mesenchymal surface markers as highly as the first passage cells grown in tissue culture dishes (24–38% vs. 53–75%). These data indicate that the purity of hADSCs in the recovery solution is approximately half that of the first passage hADSCs grown in tissue culture dishes. However, only 30 min was required to isolate hADSCs from the primary adipose tissue cell solution by the membrane filtration method, whereas 5–12 days were necessary to purify hADSCs via the culture method.

Osteogenic ability in vitro directly correlated with level of MSC purification and their surface markers (CD44, CD73 and CD90) expression. Interestingly, CD34+ cells were highly enriched in recovery solution – 20.2%, compared to 9% in SVF and 1.6% in first passage of cultured ADSC. So the progenitor/ stem cell composition of recovery fraction after membrane filtration could be unique.

I think this approach is very interesting and worth a look. By modification of polymer surface, and playing with cell adhesion we can change a cell composition of our product at point-of-care. If therapeutic potency after such enrichment would be good enough, I’d be happy to ditch ADSC expansion in culture.

{ 1 comment… read it below or add one }

guido veliz August 22, 2014 at 12:01 pm

Dear Sirs

We are interested in to know more about the paper that describes a protocol for purification of human adipose-derives stem cells (ADSCs) from SVF, which based on membrane filtration. I would a ppreciate a lot if you can tell me which company produce a product for to sell

Reply

Leave a Comment

Previous post:

Next post: