Cell Culture Basics: Cell viability and count in hemocytometer

by Alexey Bersenev on August 23, 2012 · 1 comment

in cell culture

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Counting cells with hemocytometer is the most popular post on our blog (> 3.5k views). We’re still getting some questions and comments about it.

Using hemocytometer for cell count and viability is a daily procedure for most of you. But new comers always make mistakes in chamber’s loading, count and viability assessment. We believe that video protocols is the best way to learn and teach. Today, we’ve picked the most helpful and understandable videos:

How to load hemocytometer correctly:

How to count:

This one is a bit funny, but i like it:

Don’t forget about online calculators!

Feel free to discuss any technical issues in comments. For example:
– Should I use ethanol for cleaning of hemocytometer or would water be ok too?
– Do I need to use a drop of water to place a cover slip tightly?
– What the hek is kimwipes?

{ 1 comment… read it below or add one }

wala Hemedat October 18, 2016 at 10:39 pm

Dear Sir,
I want to ask which is better to clean the hemocytometer when you are going to move from of sample for counting to another sample for counting with ethanol or with distilled water?
Actually, on of my colleague said that it is better to use distilled water because the ethanol make the the cells to stick on the hemocytometer from the previous sample thus this will affect the results for the next sample. I researched on the internet but I did not find anything.
thank you

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