Because significant part of our audience is young researchers, we will pay special attention to education materials and basic techniques. We’re starting a new series “Cell Culture Basics”. We will collect and share useful information in a format of digest or original post. Today, the first digest – biosafety cabinets (BSC) as a part of aseptic technique.
What are biosafety cabinets and cell culture hoods? What is the difference?
Biological safety cabinets, laminar flow hoods, clean hoods and culture hoods are all common names for those essential pieces of equipment that you use in cell culturing. The terms are used inter-changeably, but in fact there are lots of different types of culture hoods, each of which does a different job.
Demonstration of the difference between biological safety cabinets and simple laminar flow hood:
Very good education video about BSC with overview of common mistakes:
3 Ways to Abuse Biological Safety Cabinets (BitesizeBio):
Any disruption in airflow disturbs the protective barrier, and movement into and out of the hood show be minimized. Likewise, air vents, usually covered by grills, should never be blocked, as this will disrupt the flow of air. I can’t tell you how many times I have seen pipettes lying on top of grills!
General tips for good aseptic techniques in the BSC:
How to decontaminate BSC in GMP settings:
Please leave your feedback about this series. Feel free to contribute – share your experience and give some tips!
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