Isolation and expansion of colony-forming endothelial progenitor cells from cord blood

by Alexey Bersenev on March 6, 2012 · 0 comments

in cord blood, protocols

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Vasculegenic progenitors could have a great therapeutic value. There are few candidate cell populations with vasculo- and angiogenic potential. In order to bring these cells into the clinic, each population should be identified, characterized and expanded in clinical-grade conditions. A new study, published in Stem Cells Translational Medicine, provides a protocol for identification, isolation and serum-free culture of cord blood-derived endothelial pogenitor cells (EPC):

…despite the effective results for therapeutic strategy in many researches, EPCs remain a controversial topic among researchers as there is no definitive delineation of EPCs, no clear differentiation hierarchy, and no unambiguously defined isolation protocol. Therefore, the methodological development of EPC culture has lacked the definitive EPC evaluation methodology.

The authors defined 2 types of colonies, isolated from CD133+ EPC, and offered a protocol for expansion of those with higher vasculogenic properties in vivo.

The present study demonstrates that our novel evaluation method for serum-free expansion provides a transplantable EPC source with quantitative and qualitative advantages for cardiovascular regeneration in ischemic disease.

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