Cord blood hematopoietic cells expansion in hollow fiber bioreactor

by Alexey Bersenev on March 14, 2012 · 2 comments

in cell culture, cell product, cord blood, hematopoietic

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There is a significant clinical demand for expansion of hematopoietic cells from cord blood. We have seen many proof-of-concept studies and some clinical trials currently under way. But we’re still missing the manufacturing part. How to make it clinically grade, scalable and cost-effective? Implementation of bioreactors could address the question of scalability. Hollow fiber bioreactors (HFBR) allow to concentrate cells and minimize the media consumption.

The recent study, published in Cell Transplantation, investigates the applicability of HFBR for CD34+ cells expansion from cord blood. The authors were able to expand CD34+ progeny at cell density 30 millions/ml.. They tested different cell culture conditions with basal media.

Using this platform we direct HSCs down the myeloid lineage, while targeting a 100-fold increase in cell density and the use of protein-free bulk medium. Our results demonstrate the potential of this system to deliver high cell densities, even in the absence of protein supplementation of the bulk medium.

These results demonstrate that this culture methodology is viable. Future work will seek to extend the culture period and range of expansion by trialing lower cell seeding densities and perhaps evaluating densities greater than 108 cells/ml.

If you’re working on expansion of cord blood cells at manufacturing site, look at this study.

{ 2 comments… read them below or add one }

Jim H August 31, 2012 at 7:37 am

Alexey,

Your link to the article is broken. Do you have the complete reference?

Thanks, Jim H

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Alexey Bersenev September 2, 2012 at 9:44 am

Hi Jim,
Seem like Ingenta changed the link when article went to print version. I fixed it. Try this link –
http://www.ingentaconnect.com/content/cog/ct/2012/00000021/00000006/art00014

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