Stability of mesemchymal stromal cells after 25 years of bone marrow cryopreservation

by Alexey Bersenev on February 5, 2012 · 0 comments

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Despite, the commercial offer to store your own bone marrow for regenerative medicine purposes, we have no data on function of stem cells after long-term storage. Recently, the potency of mesenchymal stromal cells (MSC) was tested after 23-25 years of bone marrow cryopreservation:

Samples of human BMCs that were cryopreserved for 23–25 years (n = 20) were thawed to obtain an initial culture and a primary culture (P0) that was propagated through five passages (P1–P5) to obtain MSCs. Freshly collected human bone marrow samples (n = 20) were used as controls for comparison of efficiency of recovery and growth characteristics of MSCs.
The cell growth and cell cycle patterns were similar for both groups. MSCs at P3 from both groups had similar capacities to differentiate in vitro into osteoblasts, adipocytes, and neuronal cells.

The potency of MSC after cryopreservation has become important issue after Galipeau’s grop study.

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