Simple and rapid method for enrichment of stem cells from lipoaspirates

by Alexey Bersenev on November 9, 2011 · 1 comment

in adipose, cell separation, methods

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Korean scientists have discovered a very simple, rapid and cheap method for enrichment of adipose-derived stem cells from lipoaspirates. All you need to do is leave fat in refrigerator for a day! This is totally awesome guys!

This study examined a rapid isolation method decreasing the time and cost of the clinical application of adipose tissue-derived stem cells (ASCs).

When the lipoaspirates were stored at 4℃, the total yield of mononuclear cells decreased, but the stem cell population was enriched.

Our results suggest that ASCs can be isolated with high frequency by optimal storage at 4℃ for 24 hours, and those ASCs are highly proliferative and multipotent, similar to ASCs isolated from fresh lipoaspirates.

Therefore, if MSCs were isolated rapidly from adipose tissue using our procedure in numbers approximately 100+ fold than from bone marrow, ASCs may provide the best stem cells for clinical trials.

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Dmitry August 28, 2012 at 5:16 am

In our laboratory, “BioPro Stem Technology” we got the same effect. We even kept lipoaspirat 7 days at 4 degrees and retrieves mesenchymal cells of good quality and in quantity.

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