Protocols for clinical-scale expansion of cord blood hematopoietic progenitor cells

by Alexey Bersenev on August 18, 2011 · 1 comment

in cord blood, hematopoietic, protocols

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Three recent articles provide different approaches for expansion of cord blood hematopoietic progenitor and stem cells. All protocols available in open access.

1. Clinical-scale cultures of cord blood CD34+ cells to amplify committed progenitors and maintain stem cell activity

Serum-free culture with bunch of cytokines. Expansion rate: total MNC ~350 fold, CD34+ ~100 fold, CFC ~130 fold. In vivo test in serial transplant. No hematopoietic stem cells (HSC) expansion confirmed in vivo, but maintenance.

2. Preclinical transplantation and safety of HS/PCs expanded from human umbilical cord blood

Mesenchymal stem cells for niche mimicking were used. Expansion rate: total MNC ~125 fold, CD34+ ~43 fold. In vivo transplantation assay were not done properly, but indicate to increased hematopoietic recovery in “expanded cells” group.

3. Human CD34+ CD133+ hematopoietic stem cells cultured with growth factors including Angptl5 efficiently engraft adult NOD-SCID Il2rγ−/− (NSG) mice

Serum-free culture with cytokines including Angptl5 and IGFBP2. Expansion rate in vivo: CD133+/CD34+ ~21 fold, in vivo: CD133+/CD34+ ~20 fold in SCID-repopulation activity. Very well done in vivo transplantation assays. This study is highly recommended to read!

{ 1 comment… read it below or add one }

armita August 2, 2015 at 1:21 pm

hi,
would you please answer to my question,how long it takes cord blood cell growth,when we are separate cells.

thanks for your helpfull

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