I do hematopoietic stem cell homing assay myself and know all of hurdles. This protocol is really helpful.
Homing is the phenomenon whereby transplanted hematopoietic cells are able to travel to and engraft or establish residence in the bone marrow.
This paper outlines the classic homing protocol used in hematopoietic stem cell studies. In general this involves isolating the cell population whose homing needs to be investigated, staining this population with a dye of interest and injecting these cells into the blood stream of a recipient animal.
Homing of Hematopoietic Cells to the Bone Marrow
Rushdia Z. Yusuf, David T. Scadden
Center for Regenerative Medicine, Massachusetts General Hospital
This article describes a protocol used to study the homing of hematopoietic cells to their niches in the bone marrow.
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