Making an implantable tissue engineered liver using decellularized matrix

by Alexey Bersenev on February 15, 2011 · 0 comments

in tissue engineering

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We are continue to follow the recent trend in tissue engineering – using decellularized tissues and organs as a matrix. Today I’d like to share the proof of concept study for whole liver function replacement by decellularized matrix repopulated with hepatocytes:

We developed an intact 3D scaffold of an extracellular matrix (ECM) derived from a decellularized liver lobe, with layer-by-layer (LbL) heparin deposition to avoid thrombosis, which we repopulated with hepatocytes and successfully implanted as a tissue-engineered liver (TEL) into the portal system. The TEL provided sufficient volume for transplantation of cell numbers representing up to 10% of whole-liver equivalents and was perfused by portal vein blood. Treatment of extended hepatectomized rats with a TEL improved liver function and prolonged survival; mean lifespan was extended from 16 to 72 hours. At 72 hours post-operation, the TEL sustained functional and viable hepatocytes. In conclusion, we propose the TEL as a state-of-art substitute for whole-liver transplantation and as a proof of concept for the technology that will eventually allow for the transplantation of a reconstituted liver.

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