Isolation of endothelial cells with high clonogenic potential from human peripheral blood progenitors

by Alexey Bersenev on November 23, 2010 · 0 comments

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Endothelial progenitor cellls (EPC) or so-called outgrowth endothelial cells (OEC), derived from peripheral blood, cord blood or bone marrow, have promising therapeutic potential in ischaemia. I’d like to share a protocol for OEC enrichment based on cell culture modification. The protocol published in Tissue Engineering Part C: Methods in open access.

According to the current knowledge EPC are contained in very low numbers in peripheral blood and represent only 0.01% to 0.0001% of MNC. This low frequency, together with the lack of commonly accepted markers, complicates their accurate detection or directed isolation. Many of the cells termed EPC might support the neovascularization process more by paracrine or indirect and synergistic effects than by differentiation into mature and functional endothelial cells. In contrast to this, OEC are a distinct population with a promising angiogenic potential.

In this study we propose a simple modification of a standard protocol for the isolation of OEC from peripheral blood by including a passaging step in the early phase of EPC culture.

In conclusion, an enrichment of relevant cell types by protocol modification as suggested in the present study might be valuable in further improving the detection of endothelial cell colony-forming cells or their progenitors. This method might serve as an alternative or as an additional step for currently used protocols for EPC isolations.

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