Stem Cells journal has some newly published or even prepublished online articles in open access. Entire journal is for free after one year of publication. I’d recommend you to check it out.
Today I’ve picked the paper – Efficient generation of functional dopaminergic neurons from human induced pluripotent stem cells under defined conditions. This study is very well done methodologically.
We used a completely defined (xeno-free) system we previously developed for efficient generation of authentic dopaminergic neurons from human embryonic stem cells (hESCs), and applied it to iPSCs. First, we adapted two human iPSC lines derived from different somatic cell types to the defined expansion medium and showed that the iPSCs grew similarly as hESCs in the same medium regarding pluripotency and genomic stability. Second, by using these two independent adapted iPSC lines we showed that the process of differentiation into committed neural stem cells (NSCs) and subsequently into dopaminergic neurons was also similar to hESCs. Importantly, iPSC-derived dopaminergic neurons were functional as they survived and improved behavioral deficits in 6-hydroxydopamine-leasioned rats after transplantation.
Related posts:
- Protocol for generation of human induced pluripotent stem cells
- Human NK cell generation from progenitors and stem cells
- Guidelines and techniques for the generation of induced pluripotent stem cells
- Lab-specific gene expression signatures in pluripotent stem cells
- Protocol for generation of transgene-free iPS cells
