In 1980-1982 two Russian scientists – Alexander Friedenstein and Joseph Chertkov showed for the first time that bone marrow-derived stromal precursor cells able to form hematopoietic microenvironment in heterotopic transplantation experiment.
25 years later, scientists proposed and prospectively isolate hematopoietic niche cell candidates, based on achievements of flow cytometry. I would like to indicate some papers where protocols for isolation of hematopoietic niche cell candidates were published.
mouse:
1. CD45-/Ter119-/OPN+ osteoblasts Blood 2008;112:519 (open access)
2. CD105+/Thy1- fetal bone progenitors Nature 2009;457:490 | doi:10.1038/nature07547
3. ALCAM±/Sca-1± endosteal cells Blood prepublished online May 14, 2010; DOI 10.1182/blood-2009-08-239194
4. Nestin+ mesenchymal stem cells Nature 2010;466:829 | doi:10.1038/nature09262
5. PDGFRα+/Sca-1+/CD45−/Ter119− mesenchymal stem cells JEM 2009;206:2483 | doi: 10.1084/jem.20091046 (open access)
human:
CD146+ bone marrow stromal cells Cell 2007; 131:324 |doi:10.1016/j.cell.2007.08.025 (open access)
Related posts:
- Mesenchymal stem cells isolation in one step
- Isolation and culture of mesenchymal stem cells from mouse bone marrow
- Identification and isolation of hematopoietic stem cell subsets in human cord blood
- Difference in radioresistance of human and mouse hematopoietic stem cells
- Isolation of mesenchymal stem cells from mouse compact bone – video protocol
