I’ve wrote about current trends in tissue engineering methodologies a while ago. One of the most promising approaches is using decellularized cadaver organs as a matrix instead of polymer scaffolds. The recent advanced in this technique allowed to create functional bioengineered lungs.
I was looking for the freely available tissue decellularization protocol and now I can share one with you. Very good article, describing lung tissue engineering based on acellular matrix and embryonic stem cells, just out of print and available in open access.
In the “Methods” section of the article you can find detailed protocol for lungs tissue decellularization.
We report here the first attempt to produce and use whole acellular (AC) lung as a matrix to support development of engineered lung tissue from murine embryonic stem cells (mESCs). We compared the influence of AC lung, Gelfoam, Matrigel, and a collagen I hydrogel matrix on the mESC attachment, differentiation, and subsequent formation of complex tissue. We found that AC lung allowed for better retention of cells with more differentiation of mESCs into epithelial and endothelial lineages..
Study is very well done methodologically and can be used for designing your own experiments in tissue engineering.
Tissue Engineering is a journal with open access option. Please consider this journal in order to make your study freely available and reproducible around the world.
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