High-throughput transfection of human embryonic stem cells by electroporation

by Alexey Bersenev on July 26, 2010 · 0 comments

in embryonic/iPS, gene transfer

Post to Twitter Send Gmail Post to LinkedIn

I like the studies that compare 2 or more commercial products dedicated to the same method.

The authors of a new study, pre-published online in Stem Cell Research & Therapy journal, compared 2 commercial electroporation systems for transfection of human embryonic stem cells.

We investigated the ability of two commercially available electroporation systems, the Nucleofection(R) 96-well Shuttle(R) System from Lonza and the NeonTM Transfection System from Invitrogen to efficiently transfect hESC. Transfection efficiency was measured by FACS analysis for GFP and the viability of the transfected cells was determined by an ATP catalyzed luciferase reaction. The transfected cells were also analyzed by FACS for common markers of pluripotency.

full text article is available in open access!

Stem Cell Research & Therapy – new open access journal from BioMed Central.

{ 0 comments… add one now }

Leave a Comment

Previous post:

Next post: