Identification and isolation of hematopoietic stem cell subsets in human cord blood

by Alexey Bersenev on March 31, 2010 · 1 comment

in cord blood, hematopoietic, protocols

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I’d like to share very good paper, included the protocol for hierarchical characterization and isolation of hematopoietic stem cell (HSC) populations in human cord blood. This work was done in Irving Weissman lab and published in 2007 in Cell Stem Cell journal. This protocol is kind of unique, because the only one (in my knowledge) providing all details and established top hierarchy of human HSC.

Previous reports indicate that human HSC are enriched in Lin−CD34+CD38− cord blood and bone marrow and express CD90. We demonstrate that the Lin−CD34+CD38− fraction of cord blood and bone marrow can be subdivided into three subpopulations: CD90+CD45RA−, CD90−CD45RA−, and CD90−CD45RA+. Utilizing in vivo transplantation studies and complementary in vitro assays, we demonstrate that the Lin−CD34+CD38−CD90+CD45RA− cord blood fraction contains HSC and isolate this activity to as few as 10 purified cells. Furthermore, we report the first prospective isolation of a population of candidate human multipotent progenitors, Lin−CD34+CD38−CD90−CD45RA− cord blood.

full text in open access

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