Targeted gene transfer can offer you the possibility of desired gene delivery into specific cell type or tissue. In order to achieve that few modifications with viral vectors was made.
…lentiviral vectors that can target tissues specifically will be valuable for various gene-transfer approaches in vivo. To achieve targeted gene transfer, two types of surface modifications have been made to lentiviral vectors: (1) heterologous viral glycoproteins have been incorporated to exploit the tropism of other viruses (this is called pseudotyping), and (2) heterologous polypeptides have been fused to viral glycoproteins to retarget the lentiviral particles to a cell of interest.
I’d like to share a link to open access protocol – Hematopoietic stem cell targeting with surface-engineered lentiviral vectors.
In the protocol presented here, hematopoietic stem cells (HSCs) are specifically transduced with a vector displaying the HSC-activating polypeptides, stem cell factor (SCF) and thrombopoietin (TPO). Targeted HSC transduction is evaluated in the non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mouse model.
read more stem cell protocols from Cold Spring Harbor Protocols collection