Isolation and culture of mesenchymal stem cells from mouse bone marrow

by Alexey Bersenev on January 20, 2010 · 0 comments

in mesenchymal, protocols

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Nature Protocols featured Stem Cell Series. Some protocols generously provided for free. I’ll highlight some of them in following posts. Stay tuned!

Today I’d like to share free protocol: A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow published a year ago by Masoud Soleimani & Samad Nadri.

Two stem cell populations with distinct progenies are housed within adult BM, hematopoietic stem cells and MSCs. MSCs have been referred to in the literature by other names such as colony-forming fibroblastic cells, BM stromal stem cells, mesenchymal progenitor cells and BM stromal cells.

Protocol is simple and does not include antibody-based separation techniques (FACS, MACS)

…the standard and unmodified method based on plastic adherence has been confirmed as unsuccessful for mMSCs because various hematopoietic cell lineages proliferate on stromal layers and therefore constitute a large percentage of the plastic adherent population.

Consequently, we developed a method on the basis of frequent medium changes during the initial phase of culturing and diminished duration of trypsination to fractionate hematopoietic and endothelial lineages from plastic adherent fibroblastoid (stromal) cells derived from murine BM.

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